Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema
Identifieur interne : 002761 ( Main/Exploration ); précédent : 002760; suivant : 002762Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema
Auteurs : Ira L. Savetsky ; Jeremy S. Torrisi ; Daniel A. Cuzzone ; Swapna Ghanta ; Nicholas J. Albano ; Jason C. Gardenier ; Walter J. Joseph ; Babak J. MehraraSource :
- American Journal of Physiology - Heart and Circulatory Physiology [ 0363-6135 ] ; 2014.
Descripteurs français
- KwdFr :
- Adiposité, Alimentation riche en graisse, Animaux, Facteurs temps, Fibrose, Graisse sous-cutanée (physiopathologie), Granulocytes neutrophiles (immunologie), Huile de croton, Indice de gravité médicale, Infiltration par les neutrophiles, Inflammation (), Inflammation (immunologie), Inflammation (physiopathologie), Inflammation (étiologie), Lymphoedème (anatomopathologie), Lymphoedème (immunologie), Lymphoedème (physiopathologie), Lymphoedème (étiologie), Macrophages (immunologie), Modèles animaux de maladie humaine, Mâle, Obésité (), Obésité (immunologie), Obésité (physiopathologie), Phénotype, Souris, Souris de lignée C57BL, Système lymphatique (anatomopathologie), Système lymphatique (immunologie), Système lymphatique (physiopathologie).
- MESH :
- anatomopathologie : Lymphoedème, Système lymphatique.
- immunologie : Granulocytes neutrophiles, Inflammation, Lymphoedème, Macrophages, Obésité, Système lymphatique.
- physiopathologie : Graisse sous-cutanée, Inflammation, Lymphoedème, Obésité, Système lymphatique.
- étiologie : Inflammation, Lymphoedème.
- Adiposité, Alimentation riche en graisse, Animaux, Facteurs temps, Fibrose, Huile de croton, Indice de gravité médicale, Infiltration par les neutrophiles, Inflammation, Modèles animaux de maladie humaine, Mâle, Obésité, Phénotype, Souris, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Adiposity, Animals, Croton Oil, Diet, High-Fat, Disease Models, Animal, Fibrosis, Inflammation (chemically induced), Inflammation (etiology), Inflammation (immunology), Inflammation (physiopathology), Lymphatic System (immunology), Lymphatic System (pathology), Lymphatic System (physiopathology), Lymphedema (etiology), Lymphedema (immunology), Lymphedema (pathology), Lymphedema (physiopathology), Macrophages (immunology), Male, Mice, Mice, Inbred C57BL, Neutrophil Infiltration, Neutrophils (immunology), Obesity (complications), Obesity (immunology), Obesity (physiopathology), Phenotype, Severity of Illness Index, Subcutaneous Fat (physiopathology), Time Factors.
- MESH :
- chemical : Croton Oil.
- chemically induced : Inflammation.
- complications : Obesity.
- etiology : Inflammation, Lymphedema.
- immunology : Inflammation, Lymphatic System, Lymphedema, Macrophages, Neutrophils, Obesity.
- pathology : Lymphatic System, Lymphedema.
- physiopathology : Inflammation, Lymphatic System, Lymphedema, Obesity, Subcutaneous Fat.
- Adiposity, Animals, Diet, High-Fat, Disease Models, Animal, Fibrosis, Male, Mice, Mice, Inbred C57BL, Neutrophil Infiltration, Phenotype, Severity of Illness Index, Time Factors.
Abstract
Although obesity is a major clinical risk factor for lymphedema, the mechanisms that regulate this effect remain unknown. Recent reports have demonstrated that obesity is associated with acquired lymphatic dysfunction. The purpose of this study was to determine how obesity-induced lymphatic dysfunction modulates the pathological effects of lymphatic injury in a mouse model. We used a diet-induced model of obesity in adult male C57BL/6J mice in which experimental animals were fed a high-fat diet and control animals were fed a normal chow diet for 8–10 wk. We then surgically ablated the superficial and deep lymphatics of the midportion of the tail. Six weeks postoperatively, we analyzed changes in lymphatic function, adipose deposition, inflammation, and fibrosis. We also compared responses to acute inflammatory stimuli in obese and lean mice. Compared with lean control mice, obese mice had baseline decreased lymphatic function. Lymphedema in obese mice further impaired lymphatic function and resulted in increased subcutaneous adipose deposition, increased CD45+ and CD4+ cell inflammation (
Url:
DOI: 10.1152/ajpheart.00244.2014
PubMed: 24858842
PubMed Central: 4101643
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 002B92
- to stream Pmc, to step Curation: 002B91
- to stream Pmc, to step Checkpoint: 001949
- to stream PubMed, to step Corpus: 001508
- to stream PubMed, to step Curation: 001508
- to stream PubMed, to step Checkpoint: 001508
- to stream Ncbi, to step Merge: 006687
- to stream Ncbi, to step Curation: 006687
- to stream Ncbi, to step Checkpoint: 006687
- to stream Main, to step Merge: 002765
- to stream Main, to step Curation: 002761
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema</title><author><name sortKey="Savetsky, Ira L" sort="Savetsky, Ira L" uniqKey="Savetsky I" first="Ira L." last="Savetsky">Ira L. Savetsky</name></author><author><name sortKey="Torrisi, Jeremy S" sort="Torrisi, Jeremy S" uniqKey="Torrisi J" first="Jeremy S." last="Torrisi">Jeremy S. Torrisi</name></author><author><name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name></author><author><name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name></author><author><name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name></author><author><name sortKey="Gardenier, Jason C" sort="Gardenier, Jason C" uniqKey="Gardenier J" first="Jason C." last="Gardenier">Jason C. Gardenier</name></author><author><name sortKey="Joseph, Walter J" sort="Joseph, Walter J" uniqKey="Joseph W" first="Walter J." last="Joseph">Walter J. Joseph</name></author><author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24858842</idno><idno type="pmc">4101643</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101643</idno><idno type="RBID">PMC:4101643</idno><idno type="doi">10.1152/ajpheart.00244.2014</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002B92</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002B92</idno><idno type="wicri:Area/Pmc/Curation">002B91</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002B91</idno><idno type="wicri:Area/Pmc/Checkpoint">001949</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001949</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">001508</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001508</idno><idno type="wicri:Area/PubMed/Curation">001508</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001508</idno><idno type="wicri:Area/PubMed/Checkpoint">001508</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001508</idno><idno type="wicri:Area/Ncbi/Merge">006687</idno><idno type="wicri:Area/Ncbi/Curation">006687</idno><idno type="wicri:Area/Ncbi/Checkpoint">006687</idno><idno type="wicri:doubleKey">0363-6135:2014:Savetsky I:obesity:increases:inflammation</idno><idno type="wicri:Area/Main/Merge">002765</idno><idno type="wicri:Area/Main/Curation">002761</idno><idno type="wicri:Area/Main/Exploration">002761</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema</title><author><name sortKey="Savetsky, Ira L" sort="Savetsky, Ira L" uniqKey="Savetsky I" first="Ira L." last="Savetsky">Ira L. Savetsky</name></author><author><name sortKey="Torrisi, Jeremy S" sort="Torrisi, Jeremy S" uniqKey="Torrisi J" first="Jeremy S." last="Torrisi">Jeremy S. Torrisi</name></author><author><name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name></author><author><name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name></author><author><name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name></author><author><name sortKey="Gardenier, Jason C" sort="Gardenier, Jason C" uniqKey="Gardenier J" first="Jason C." last="Gardenier">Jason C. Gardenier</name></author><author><name sortKey="Joseph, Walter J" sort="Joseph, Walter J" uniqKey="Joseph W" first="Walter J." last="Joseph">Walter J. Joseph</name></author><author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name></author></analytic><series><title level="j">American Journal of Physiology - Heart and Circulatory Physiology</title><idno type="ISSN">0363-6135</idno><idno type="eISSN">1522-1539</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adiposity</term><term>Animals</term><term>Croton Oil</term><term>Diet, High-Fat</term><term>Disease Models, Animal</term><term>Fibrosis</term><term>Inflammation (chemically induced)</term><term>Inflammation (etiology)</term><term>Inflammation (immunology)</term><term>Inflammation (physiopathology)</term><term>Lymphatic System (immunology)</term><term>Lymphatic System (pathology)</term><term>Lymphatic System (physiopathology)</term><term>Lymphedema (etiology)</term><term>Lymphedema (immunology)</term><term>Lymphedema (pathology)</term><term>Lymphedema (physiopathology)</term><term>Macrophages (immunology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Neutrophil Infiltration</term><term>Neutrophils (immunology)</term><term>Obesity (complications)</term><term>Obesity (immunology)</term><term>Obesity (physiopathology)</term><term>Phenotype</term><term>Severity of Illness Index</term><term>Subcutaneous Fat (physiopathology)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adiposité</term><term>Alimentation riche en graisse</term><term>Animaux</term><term>Facteurs temps</term><term>Fibrose</term><term>Graisse sous-cutanée (physiopathologie)</term><term>Granulocytes neutrophiles (immunologie)</term><term>Huile de croton</term><term>Indice de gravité médicale</term><term>Infiltration par les neutrophiles</term><term>Inflammation ()</term><term>Inflammation (immunologie)</term><term>Inflammation (physiopathologie)</term><term>Inflammation (étiologie)</term><term>Lymphoedème (anatomopathologie)</term><term>Lymphoedème (immunologie)</term><term>Lymphoedème (physiopathologie)</term><term>Lymphoedème (étiologie)</term><term>Macrophages (immunologie)</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Obésité ()</term><term>Obésité (immunologie)</term><term>Obésité (physiopathologie)</term><term>Phénotype</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Système lymphatique (anatomopathologie)</term><term>Système lymphatique (immunologie)</term><term>Système lymphatique (physiopathologie)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Croton Oil</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Lymphoedème</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="chemically induced" xml:lang="en"><term>Inflammation</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>Obesity</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Inflammation</term><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Granulocytes neutrophiles</term><term>Inflammation</term><term>Lymphoedème</term><term>Macrophages</term><term>Obésité</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Inflammation</term><term>Lymphatic System</term><term>Lymphedema</term><term>Macrophages</term><term>Neutrophils</term><term>Obesity</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lymphatic System</term><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Graisse sous-cutanée</term><term>Inflammation</term><term>Lymphoedème</term><term>Obésité</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Inflammation</term><term>Lymphatic System</term><term>Lymphedema</term><term>Obesity</term><term>Subcutaneous Fat</term></keywords><keywords scheme="MESH" qualifier="étiologie" xml:lang="fr"><term>Inflammation</term><term>Lymphoedème</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adiposity</term><term>Animals</term><term>Diet, High-Fat</term><term>Disease Models, Animal</term><term>Fibrosis</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Neutrophil Infiltration</term><term>Phenotype</term><term>Severity of Illness Index</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adiposité</term><term>Alimentation riche en graisse</term><term>Animaux</term><term>Facteurs temps</term><term>Fibrose</term><term>Huile de croton</term><term>Indice de gravité médicale</term><term>Infiltration par les neutrophiles</term><term>Inflammation</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Obésité</term><term>Phénotype</term><term>Souris</term><term>Souris de lignée C57BL</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Although obesity is a major clinical risk factor for lymphedema, the mechanisms that regulate this effect remain unknown. Recent reports have demonstrated that obesity is associated with acquired lymphatic dysfunction. The purpose of this study was to determine how obesity-induced lymphatic dysfunction modulates the pathological effects of lymphatic injury in a mouse model. We used a diet-induced model of obesity in adult male C57BL/6J mice in which experimental animals were fed a high-fat diet and control animals were fed a normal chow diet for 8–10 wk. We then surgically ablated the superficial and deep lymphatics of the midportion of the tail. Six weeks postoperatively, we analyzed changes in lymphatic function, adipose deposition, inflammation, and fibrosis. We also compared responses to acute inflammatory stimuli in obese and lean mice. Compared with lean control mice, obese mice had baseline decreased lymphatic function. Lymphedema in obese mice further impaired lymphatic function and resulted in increased subcutaneous adipose deposition, increased CD45<sup>+</sup> and CD4<sup>+</sup> cell inflammation (<italic>P</italic> < 0.01), and increased fibrosis, but caused no change in the number of lymphatic vessels. Interestingly, obese mice had a significantly increased acute inflammatory reaction to croton oil application. In conclusion, obese mice have impaired lymphatic function at baseline that is amplified by lymphatic injury. This effect is associated with increased chronic inflammation, fibrosis, and adipose deposition. These findings suggest that obese patients are at higher risk for lymphedema due to impaired baseline lymphatic clearance and an increased propensity for inflammation in response to injury.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name><name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name><name sortKey="Gardenier, Jason C" sort="Gardenier, Jason C" uniqKey="Gardenier J" first="Jason C." last="Gardenier">Jason C. Gardenier</name><name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name><name sortKey="Joseph, Walter J" sort="Joseph, Walter J" uniqKey="Joseph W" first="Walter J." last="Joseph">Walter J. Joseph</name><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name><name sortKey="Savetsky, Ira L" sort="Savetsky, Ira L" uniqKey="Savetsky I" first="Ira L." last="Savetsky">Ira L. Savetsky</name><name sortKey="Torrisi, Jeremy S" sort="Torrisi, Jeremy S" uniqKey="Torrisi J" first="Jeremy S." last="Torrisi">Jeremy S. Torrisi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002761 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002761 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:4101643
|texte= Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24858842" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |